Universitas Indonesia Conferences, 1st International Conference on Advance Pharmacy and Pharmaceutical Sciences

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Nephroprotective effect of Pterocarpus indicus Willd. leaves : observation from plasma urea and creatinine levels against gentamicin-induced nephrotoxicity in Sprague-Dawley rats
Fikry Dwi Anjani, desy rasta waty, Fadlina Chany saputri

Last modified: 2016-09-12


Pterocarpus indicus Willd. has been widely used empirically for treating canker sore, antibacterial and kidney disease. This study aimed to evaluate nephroprotective effect water-boiled P.indicus leaves against gentamicin-induced  nephrotoxicity in rats observed from plasma urea and creatinine levels. In this study, thirty (30) male rats divided into five treatment groups. The treatment groups consisted  of normal group,  induction group received gentamicin (80 mg/kg body weight per day i.p.) and  group which received water-boiled P.indicus leaves with a dose of  28.8 mg/kg, 57.6 mg/kg, and 115.2 mg/kg body weight per day p.o. All groups received treatment for 21 days. At the 15th day, the animals received gentamicin i.p. for 7 days. At the 22nd day, the blood was collected from orbital sinus. The plasma urea and creatinine levels were measured by Berthelot method for plasma urea and colorimetric method for plasma creatinine. The highest dose of  115.2 mg/kg water-boiled P.indicus was decreased  plasma urea and creatinine levels also significantly different (p<0,05) compared induction group. Water-boiled P.indicus leaves have a potential to prevent gentamicin-induced nephrotoxicity might be due to  its  antioxidant and free radical scavenging activity.


Keywords: gentamicin, plasma creatinine, plasma urea, Pterocarpus indicus Willd,  nephroprotective.

Abbreviation: WPIL : Water-boiled Pterocarpus indicus Leaves, i.p. : Intraperitoneal Injection, p.o. : Per Oral.