Universitas Indonesia Conferences, 1st International Conference on Advance Pharmacy and Pharmaceutical Sciences

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Antioxidant, anti wrinkle and tyrosinase inhibitor of brown seaweed (Padina australis)
Arief Nurrochmad

Last modified: 2016-08-24


Background: Fucoxantin from brown seaweed has been reported to suppress the expression of mRNA associated with melanogenesis skin, reducing melanogenesis factor at the level of transcription. Additionally, fucosantin been shown to have photoprotective properties in human fibroblast cells by inhibiting DNA damage and increase antioxidant activity. Padina australis is one of brown seaweed that contained fucoxanthin. Objective: This study aims to determine the activity of antioxidant and anti-aging of extracts (EPA) and fractions (FPA) of P.australis. Method: The antioxidant capacity were measured using, β-carotene bleaching assay (BCB) and ferric reducing antioxidant power (FRAP). Inhibiting effect on skin degradation enzymes was carried out using elastase and collagenase assays. The skin whitening effect of ETD and FTD were determined by tyrosinase inhibitory assay. Result: the results showed that the antioxidant activity of extracts (EPA) and fractions (FPA) of P.australis were FRAP: FCX > FPA > EPA; and BCB: BHT > EPA > FPA. Anti-wrinkles of extract and fraction of P. australis showed better activity against elastase and collagenase enzymes compare to elastantinal and NNGH (N-Isobutyl-N-(4-methoxyphenylsulfonyl). The anti-melanogenic activity showed that EPA, FPA and kojic acid have similar inhibition toward tyrosinase enzyme. The results of the present study can be concluded that extracts and fractions P.australis effective as an antioxidant action and effective as an anti-aging by inhibiting the activity of tyrosinase enzyme, elastase and collagenase. These results suggest that P.australis has high potential as a cosmetic agent due to their activities.


Keywords: Padina australis, fucoxanthin, antioxidant, anti-wrinkles, skin whitening