Universitas Indonesia Conferences, 1st International Conference on Advance Pharmacy and Pharmaceutical Sciences

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Formulation of Ethosomal Green Tea (Camellia sinensis L. Kuntze) Leaves Extract Gel and In Vitro Penetration Test Using Franz Diffusion Cell
Goldie Aisha Wirarti

Last modified: 2016-11-04


Transdermal nanovesicles dosage forms have been widely used for natural products delivery. One of the natural products that has many benefits is Green tea leaves. Benefits of Green tea leaves can be used to maintain health and cosmetics. Epigallocatechin gallat (EGCG) which is the largest catechin content of Green tea leaves is hydrophilic. Therefore, the need to improve its penetration using lipid nanovesicle, namely ethosome is needed. In addition, ethosome can also improve the stability of EGCG. This study aims to prepare and characterize Green tea leaves extract ethosome and formulate them into a gel, and a control gel prepared without ethosome. Both gels were evaluated and penetration tested using Franz diffusion cells with the skin of Sprague Dawley rats. Etosom were formulated with different concentrations, equal to 1% (F1), 1.5% (F2), and 2% (F3) of EGCG. Based on the results, F1 has spherical morphology, Dmean volume value 90.53 ± 0.32 nm, polydispersity index 0.05 ± 0.00, zeta potential at -62.6 ± 5.05 mV, and the highest percentage of drug entrapped (54.39 ± 0.03 %). The cumulative amount of EGCG penetrated for ethosomal gel and and extract gel were 1364.28 ± 56.32 µg/ cm2 and 490.17 ± 2.60 µg/ cm2, respectively. With a flux value of ethosomal gel and extract gel were 56.97 ± 2,06 µg.cm-2.hour-1 and 31.09 ± 0.29 µg.cm-2.hour-1, respectively. Based on these results it can be concluded that the ethosomal gel made from F1 can increase the amount of EGCG that was penetrated.