Universitas Indonesia Conferences, 1st International Conference on Advance Pharmacy and Pharmaceutical Sciences

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Antioxidant Activity and Lipoxygenase Enzyme Inhibition Assay with Total Flavonoid Content from Garcinia lateriflora Blume Leaves Extract
Sismita Avelia

Last modified: 2016-11-04


Garcinia lateriflora Blume has been reported have antioxidant activity using DPPH (2,2-Diphenyl-1-picrylhydrazyl) method with IC50 of methanol, ethyl acetate and n-hexane leaves extract were 6.18; 8.03; and 156.8 µg/mL, repectively. Meanwhile, there has been no literature which stated have done research about lipoxygenase inhibition activity by this plant. The aim of this study is to determine the potential antioxidant activity and lipoxygenase inhibition activity from three leaf extract of Garcinia lateriflora Blume. Test methods cover, antioxidant activity assessed using FRAP (Ferric Reducing Antioxidant Power) method, in vitro lipoxygenase inhibition activity, qualitative analysis of flavonoid using thin layer chromatography and total flavonoid content using AlCl3 colorimetric method of the most active extract. The results showed that EC50for antioxidant activity using FRAP method of methanol, ethyl acetate and n-hexane Garcinia lateriflora Blume leaves extract were 9.567; 16.555; and 50.550 µg/mL and IC50for lipoxygenase inhibition activity were 0.693; 0.793; and 1.316 µg/mL, respectively. The most active extract on both of test is methanol extract which has total flavonoid content, 6.298 mg QE/g (quercetin equivalent). Based on test results can be concluded Garcinia lateriflora Blume leaves extract has antioxidant and lipoxygenase inhibition activities, with methanol extract as most active extract that contains most flavonoid.