Background: Mountain papaya (Vasconcellea pubescens A.DC.) and bitter melon (Momordica charantia L.) fruit extract have antidiabetic and antioxidant activities because of total flavonoids from its contains. Objective: This study aimed to prove the efficacy of the combination of both these plants by examining in vivo antidiabetic and antioxidant activity model in rat experimentally. Materials and Methods: Rat were divided into eight groups (normal, negative control, glibenclamide as a positive control, and five dose extract group). The dose mountain papaya extract (MPE) and bitter melon extract (BME) were given at 174 mg/kg b.w of MPE, 380 mg/kg b.w of BME, combination of MPE:BME (87:190; 44:285 and 130:95) mg/kg b.w. Induction of diabetes used alloxan dose of 150 mg/kg b.w intraperitoneally. The treatment was performed for 21 days with a frequency of once a day. Blood sugar level was measured at pretest, 7^th, 14^th, and 21^st days. At the end of the test, measurements of malondialdehyde (MDA), glutathione (GSH), and pancreatic morphology were measured. Data were analyzed by ANOVA statistic. Results: The Combination of MPE-BME at dose of 87:190; 44:285 and 130:95 mg/kg b.w /day orally, showed an decrease in blood glucose levels (58.04; 57.02 and 45.94%) than single extract MPE (45.50%) BME (54.72%) significantly (p ≤ 0.05) compared with the negative control. The same results from measurements of levels of malondialdehyde (MDA), glutathione (GSH), and pancreatic morphology. Conclusion: Based on these results, a combination of the CFE-BFE had antidiabetic and antioxidant activity, but the activity was not significantly different from the single extract (p> 0.05).