ALPHA MANGOSTIN ACTIVITY ON LANGERHANS ISLET

IN ALLOXAN INDUCED DIABETIC MICE

Welly Ratwita¹, Elin Yulinah Sukandar², I Ketut Adnyana², Neng Fisheri K².

¹⁾Laboratorium Farmakologi Fakultas Kedokteran Unjani

²⁾Laboratorium Farmakologi-Farmasi Klinik Sekolah Farmasi ITB

Corresponding author: wellyratwita@gmail.com

ABSTRACT

Background: Alpha mangostin, which was isolated from Garcinia mangostana L., have been shown antiadiabetic effect invivo by lowering blood glucose in diabetic mice induced by alloxan monohydrate. Objective: This research elaborated role of alpha mangostin  on langerhans islet in alloxan induced diabetic mice. Methods: Langerhans islet test were conducted using male Mus musculus mice, divided into 7 groups randomly, which were normal, control (alloxan induced only), glibenclamide (0.65 mg/kgbw) and metformin (65 mg/kgbw), various doses of α-mangostin (5, 10, 20 mg/kgbw). Mice were treated for 21 days. Overnight-fasted mice (12 h) were sacrificed by cervical decapitation on day 21 st , following the ethical norms granted by the ethics committee. Pancreatic tissues were excised from sacrificed animals, and then fixed in 10 % (v/v) neutral buffered formalin. Histologic observations for Langerhans area were performed after staining using Gomori staining method. Results: Langerhans area showed significant difference between alpha mangostin to control group. But there’s still had significant difference if we compare to glibenclamide/metformin group. Conclusion: Alpha mangostin showed antidiabetic effect on Langerhans islet after 21 days treatment

Keywords: α-mangostin, diabetic mice, Langerhans islet