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Acrylamide (AA) is a carcinogenic substance that is mentioned by IARC as probable human carcinogen, group 2A. This compound can be found in heated starchy food as the result of Maillard reaction between asparagine and reducing sugar. This compound is also found in tobacco smoke. Acrylamide content in cigarettes mainstream smoke was 679.3 ng/cigarette with estimated average exposure to acrylamide from tobacco smoke in adult smokers of 0.17 µg/kg b.w./day. This analysis was performed by using 90 subjects which were grouped into three groups: negative blank, smoker and non-smoker lung cancer patient. The samples were taken using dried blood spot (DBS) biosampling method. The quantitative analysis of acrylamide content in blood was determined by Ultra High Performance Liquid Chromatography tandem Mass Spectrometry (UHPLC-MS/MS) and the DBS extraction using Protein Precipitate Technique (PPT). A significant increase of acrylamide content was observed for most lung cancer patient smokers that corresponded to an uptake of acrylamide from about 3 cigarettes per day.