Background:Okra fruit has phytosterol, pectin that has the potential as an anticholesterol. Objective:The purpose of this study was to obtain  70% ethanol extract and nanoparticles of okra fruit extract and to evaluate their activity of anticholesterol. Material and Methods:Okra fruit was extracted by kinetic maceration and the produced extract was tested for phytochemical screening and anticholesterolemia activity in vivo. The nanoparticles of okra fruit extract was prepared using a cosolvention method. In this study, mice DDY strain was divided into 5 groups, namely control of normal, negative, positive (Simvastatin) and ethanolic extract 70% okra fruit (400 mg / kgBB), and the nanoparticles (~ 400 mg / kgBB). All groups except normal controls were fed 14 days with high cholesterol diet. After 14 days, the induction of hypercholesterolemia was stopped and continued with Simvastatin administration for positive control and the test groups were treated with the extract and the nanoparticles for 7 days. The nanoparticles used demonstrated particle size of 134.7 nm and polydispersity index of 0.512 and zeta potential value of -26.72. Measurement of total cholesterol level was carried out by cholesterol stripe test method. Results:The results showed a decrease in total cholesterol levels in positive control, the extract and the nanoparticles  as many as 48.68%; 32.44% and 42.95%, respectively. Conclusion:Accordingly, the nanoparticles can increase the activity of the extract.

Keywords: okra fruits, Abelmoschus esculentus L. Moench, hypercholesterolemia, total cholesterol level, nanoparticles.