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ABSTRACT

The aim of this study was to determine the effect of the ratio of solid lipids (a combination of beeswax-cacao oleum) and liquid lipids (olive oil) to the physical characteristics of nanostructured lipid carriers (NLC) as a delivery system for p-metoxisinamicic acid (PMCA). Ratio of solid lipids: liquid lipid used in this study are 60:40 in Formula 1 (F1); 70:30 in Formula 2 (F2) and 80:20 in Formula 3 (F3). The NLC-PMCA were produced by the High Shear Homogenisation method. The physical characteristics measured include; PH value, viscosity, particle size, melting point, recrystallization index and entrapment efficiency (EE). The results showed the pH value of F1, F2 and F3 was 4.29 ± 0.032; 4,33 ± 0,052; and 4.37 ± 0.014 respectively. The viscosity value is 184.53 ± 5.46; 216.87 ± 4.96; and 371.83 ± 9.83 cPs respectively. The particle size was 398.7 ± 31.66; 596.0 ± 42.31; 626.5 ± 88.18 nm repeatedly. The melting point is 67.86 ^oC (F1); 59.94 and 105.04 ^oC (F2); 56.83 and 104.15 ^oC (F3). The recrystallization index is 0.13 (F2) and 0.24% (F3). The entrapment efficiency is 36.95 ± 3.25; 29.33 ± 2.82; and 23.09 ± 1.52% respectively. Data were analyzed by one-way ANOVA method and all characteristic parameters except the pH values of each formula gave significant differences. Conclusion: a decrease in the concentration of liquid lipid (olive oil) decreases of viscosity value, particle size, and recrystallization index, but increased entrapment efficiency.   

Key words: Beeswax, Nanostructured lipid carrier (NLC), Oleum cacao, Olive oil, p-metoxycinnamic acid (PMCA), Physical characteristic.