Moringa oleifera Lam. activities have been reported in vivo. But studies that focus on adipocyte cells and its molecular have not been widely supported. This study aims to study the importance of Moringa oleifera leaf etanol extract on PPARγ gene expression and its ability to induce adipogenesis in 3T3-L1 cells. These 3T3-L1 cells were cultures in the DMEM medium stimulated by IBMX, dexamethasone, insulin, and FBS as inductors to differentiate into adipocyte cells. Confirmation of cells differentiation was done by Oil Red O staining. The effect of Moringa oleifera leaf ethanol extract was observed by adding the extract to differentiated cells and incubate for 1 day. Cells were then harvested, mRNA of PPARγ was isolated, and cDNA synthesis was carried out using qRT-PCR method. Meanwhile, adipogenesis was measured using spectrophotometry by adding Oil Red O then dye extract. Cells that are treated with Moringa oleifera leaf ethanol extract are compared with cell control and quercetin. The results showed that Moringa oleifera leaf ethanol extract increase PPARγ gene expression 70-100% and adipogenesis 30-40% greater significantly compared to cell control that was not given treatment. However, PPARγ gene expression in Moringa oleifera leaf ethanol extract is lower than quercetin and adipogenesis is higher than quercetin. Effect of Moringa oleifera leaf ethanol extract increase PPARγ gene expression and also stimulates adipogenesis, therefore it can be considered as antidiabetic agent.

Keyword: Moringa oleifera Lam., 3T3-L1 cells, adipogenesis, PPARγ gene, antidiabetic