Last modified: 2019-06-16
Abstract
Background: Plantago major has been empirically used for wound healing. One of its major chemical compounds is oleanolic acid. Objectives: This study was aimed to evaluate the diabetic wound healing mechanisms of oleanolic acid in fibroblast proliferation and migration, as well as anti-inflammation in vitro. Materials and Methods: Cell proliferation was carried out on human fibroblast cells (3T3). Rat M-24 ginggiva fibroblast was used in migration test, while anti-inflammation effect was evaluated on mouse macrophage cells (RAW264.7). these cells were cultured in high glucose medium to mimic the diabetes condition and different concentrations of oleanolic acid were subjected to the cultured cells. Results: Oleanolic acid at the concentration of 6.25-100 ppm could not increase the 3T3 proliferation. However, oleanolic acid could significantly increase the cell migration at the concentration of 25-100 ppm. Furthermore, significant inhibition of nitric oxide (NO) production was found in oleanolic acid-treated macrophage cells, suggesting its anti-inflammatory activity. Conclusion: From this study, it can be concluded that oleanolic acid could improve the diabetic wound healing process through the actions of tissue migration and anti-inflammation.
Key words: Oleanolic acid, Diabetec wound, proliferation, migration, anti-inflammatory, NO production.