Introduction: CAUTI is reported as the most common healthcare-associated infection which accounts for 30% of HAI in the hospitals. Nurses play primary role in urinary catheter care, hence determining factors influencing nurses’ practice may provide information to prevent incidences of CAUTI.

Objective: To assess factors influencing nurses’ perceived practice towards prevention of catheter-associated urinary tract infection (CAUTI).

Methodology: A cross-sectional study was conducted on a random sample of 301 registered nurses from medical and surgical wards of a large teaching hospital. Data collected using self-administered questionnaire were analyzed using univariate and multivariate analysis with SPSS version 23.

Results: The results showed that 69.1% (n=208) of the nurses had good knowledge, 80.1% (n=241) had good attitude and 64.8% (n=195) had good perceived practice towards CAUTI prevention. Significant association was noted between nurses’ knowledge level and demographic characteristics (age, gender, level of nursing education, years of experience). There were significant positive relationship between nurses’ knowledge, attitude and perceived practice.   Hierarchical multiple regression models suggested that attitude was the main predictor (37% of 44.1% of variance explaining perceived practice level) influencing nurses’ perceived practice.

Conclusion: Overall, the nurses had good knowledge, attitude and perceived practice towards CAUTI prevention. This study highlighted that attitude was the important factor that influenced nurses practice, thus measures to foster nurses’ attitude are essential rather than enhancing knowledge alone.