The antioxidant property of propolis is valuable in the pharmaceutical industry. However, the application of propolis in pharmaceutical compounds is hampered by its characteristics of low water solubility, sticky physical appearance, bitter taste, and aroma. This research aimed to optimize the microencapsulation of propolis ethanolic extract (PEE) to obtain spray-dried propolis (SDP) by using response surface methodology (RSM). The microencapsulation was use maltodextrin and gum arabic for microwalls of microcapsules with spray drying process. RSM was applied for the optimization of microencapsulation efficiency, yield, moisture content, solubility in water, total phenolic content (TPC), and antioxidant activity of SDP microcapsules. Microencapsulation optimization of propolis ethanolic extract from Tetragonula spp. using Response Surface Methodology indicated that SDP with 1:1 ratios of the core to microwall, inlet temperature at 110 ^oC and flow rate 25 % represented the optimum conditions of the microencapsulation with spray drying process. The highest process efficiency reached a microencapsulation yield of 75.35%. The highest solubility of SDP in water was 91.47%, with a moisture content of 0.96%. SDP exhibiting the highest TPC of 307.325 mg GAE/g, with a microencapsulation efficiency of 81.48%. Ferric reducing antioxidant power analysis showed its highest antioxidant activity with a low EC₅₀ 19.12 ug/ml with DPPH analysis, and a high reducing power capacity of 314.64 mg GAE/g. Microencapsulation improved physical appearance and the solubility index and protected and enhanced bioactive compounds and antioxidant properties of propolis.