Sustainable Development Goal (SDG) number six is concerning clean water and sanitation. This water specific goal includes water use efficiency and water stress. Water stress is the lack of ability to meet human and ecological demand for water. SDG Indicator 6.4.2, the level of water stress is defined as the ratio between total freshwater withdrawn by major economic sectors and total renewable freshwater resources, after subtracted with environmental water requirements. There are already map of the world water stress at country level, but for smaller unit of river basins and provinces are not yet available. As integrated water resources management recommends the water management at river basin level, the information on water stress level at river basin level should be made available. This paper calculates the SDG Indicator 6.4.2 for river basins in Java Island, Indonesia. The water stress indicator formula is following the original FAO formulation, but the environmental flow requirement is estimated using dependable flow of 95% according to the regulation in Indonesia. It is concluded that the water stress level for most of the river basins in Java Island are ranging from score 2 (from 5) of “engaged” to score 3 of “capable” stage, except the better score 4 of “effective” stage is for undeveloped river basin in the southern part of West Java, and the worst score 1 of “hazardous” stage is for Ciliwung-Cisadane river basin containing the Capital City of Jakarta with very high domestic municipal and industrial water demand.