Universitas Indonesia Conferences, 1st International Conference on Advance Pharmacy and Pharmaceutical Sciences

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Antioxidant Activity and Lipoxygenase Inhibition Test with Total Flavonoid Content from Garcinia kydia Roxburgh Leaves Extract
Nur Laily Putri

Last modified: 2016-11-11


Antioxidant is one of the therapeutic strategy to overcome oxidative stress and inhibit synthesis of inflammatory mediators through lipoxygenase pathway. Garcinia is the largest of Clusiaceae family which has been proven to provide antioxidant and anti-inflammatory activity. Garcinia kydia Roxburgh is one of the plant of this genus which is known to have antioxidant activity but lipoxygenase inhibition activity from this plant was unknown. This study aimed to test antioxidant activity of the methanol, ethyl acetate and n-hexane extract from Garcinia kydia Roxburgh leaves by FRAP (Ferric Reducing Antioxidant Power) method, anti-inflammatory activity was tested by inhibit lipoxygenase, and total flavonoid content by colorimetric methods AlCl3. The results showed antioxidant activity of methanol extract, ethyl acetate and n-hexane leaves of Garcinia kydia Roxburgh have EC50 value, respectively 18,448; 12,389 and 31,260 µg/mL, and the lipoxygenase inhibition activity have IC50 value, respectively 0,556; 0,212 and 3,575 µg/mL. Ethyl acetate extract of Garcinia kydia Roxburgh leaves were the most active extract in this study which has total flavonoid content, 30,650 mgQE/gram extract. The conclusion, Garcinia kydia Roxburgh has antioxidant and lipoxygenase inhibition activity, with ethyl acetate extract as the most active extract which contain the most total flavonoids.