Asiaticoside from gotu kola leaves extract (Centella asiatica L. Urban) could be used for anti-aging cosmetics. Nevertheless, its skin penetration is difficult, since it has a high molecular weight. Transfersome is a suitable carrier system that can enhance the penetration of active substances. The aims of this study were to prepare and characterize transfersomes of gotu kola leaves extract and formulated them into a gel, a control gel were also prepared without transfersome. The gels were evaluated for entrapment efficiency, particles morphology, particles size, polydispersity index, zeta potensial, deformability index, and penetration study using Franz diffusion cells. Transfersomes were formulated with different concentration of active substance; equals of asiaticoside 0,3%(F1), 0,5%(F2), and 0,7%(F3). The F1 transfersomes were incorporated into gel dosage form, since the F1 transfersomes had spherical morphology, the highest entrapment efficiency 85.80 ± 0.22%, Dmean volume 124.62 ± 0.86 nm, polydispersity index 0.125 ± 0.008, zeta potensial -36,3 ± 0,30 mV and deformability index 1.12. The cumulative amount of penetrated asiaticoside was 1050.85 ± 19.82 μg/cm2 for transfersomes gel and 540.21 ± 12.28 μg/cm2 for control gel. The cumulative percentage of penetrated asiaticoside for transfersomes gel and control gel were 51.80 ± 0.97% and 26.63 ± 0.60%, respectively. The flux of transfersomes gel containing asiaticoside and control gel were respectively 47.92 ± 1,74 μg/cm2/hours and 26.57 ± 0.77 μg/cm2/hours. Based on these results it can be concluded that transfersomes gel containing gotu kola leaves extract had a better penetration compared to the control gel.