Universitas Indonesia Conferences, 1st International Conference on Advance Pharmacy and Pharmaceutical Sciences

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Optimization of Microwave-Assisted Extraction of Active Compounds, Antioxidant Activity and Angiotensin Converting Enzyme (ACE) Inhibitory Activity from Peperomia pellucida (L.) Kunth
Ririn Setyaningsih

Last modified: 2016-11-03


Peperomia pellucida (L.) Kunth is one of the potential medical plants that used for the treatment of hypertension. Chemical constituens contained in this plant are phenolics, alkaloids, flavonoid, sapponins tannins. In this study, Microwave-Assisted Extraction (MAE) method was developed to obtain the optimum extraction conditions of total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AA) and angiotensin converting enzyme (ACE) inhibitory activity from Peperomia pellucida (L.) Kunth, each of which was measured by Folin-Ciocalteu method, AlCl3 method, DPPH and FRAP methods, and ACE kit-WST. MAE efficiency parameters used were ethanol concentration, sample to solvent ratio, extraction time, and microwave power.The optimum conditions of MAE for TPC (49,78 mg GAE/g extract) and TFC (37.18 mg QE/g extract) were 80% ethanol, sample-solvent ratio of 1:12, extraction time of 2 min, MAE power of 30% and 80% ethanol, ratio of 1:12, time of 2 minutes, and 70% power respectively. The optimum AA were obtained at 65% ethanol, ratio of 1:10, time of 3 min,50% power (DPPH method), and65% ethanol, ratio of 1:12, time of 1 min, and 70% power (FRAP method) respectively. The optimum ACE inhibitory activity (54.73% at a concentration of 100 µg/mL) were obtained at 80% ethanol, ratio of 1:12, time of 2 min, and 70% power. The analysis of Pearson correlation indicated that there were no correlation between TPC with FRAP method; TPC with ACE inhibitory activity and TFC with DPPH method.