More than 40% of plant compounds with beneficial biological effects are poorly soluble in water with fast elimination time and these cause bioavailability problem. One strategy to overcome this problem is formulating those compounds as nanosuspensions.  Nanosuspensions are nanocrystals of drugs dispersed in aqueous medium and stabilized with stabilizers either surfactants, polymers or combination. Two factors determine the ability to produce small and stable nanosuspensions: stabilizer concentration and duration of milling. Poloxamer 407 is a block co-polymer that can act as stabilizer with sustained release effect. This research is aimed to investigate the influence of poloxamer 407 concentrations and milling time to produce smallest and stable nanosuspensions of plant compounds. Curcumin, hesperetin and artesunate are used as plant compounds model. 0.5g powder of plant compound was dispersed in 10mL solution of Poloxamer 407 (concentration 15%, 25% and 50% w/v) and milled up to 48 hours using milling media. Sample was taken every 5, 24 and 48 hours then analyzed for its size using Photon Correlation Spectroscopy. Stability of nanosuspensions was examined by analyzing its particle size after two weeks storage at 8^oC. Results showed that the smallest particle size of hesperetin , curcumin and artesunate obtained were 141 nm (poloxamer 407 50% w/v 48 hours milling), 93 nm (poloxamer 407 25% w/v 48 hours milling) , and 218 nm (poloxamer 407 15% 24 hours milling),respectively. Samples were stable up for 2 weeks except for artesunate which showed small crystals formation with particle size increasing >50 nm.