Peperomia pellucida (L.) Kunth. herb is a wild plant that can be found in various shaded and damp habitats in tropical and subtropical climate. This plant is traditionally used as a medicine in Indonesia such as dizziness, headache, fever, and stomach ache. This study aims to determine the content of secondary metabolites and acute toxicity of methanol extracts of Peperomia pellucida (L.) Kunth herb. The methanol extract obtained by gradual maceration using n-hexane and methanol. Methanol extract solution was evaporated to have concentrated extract. Secondary metabolites analysis by thin-layer chromatography and appropriate reagents showed that methanol extract contains flavonoids, tannins, and steroids. Acute toxicity tests performed in DDY mice showed that methanol extract LD₅₀ is greater than 4000 mg/kgBW. There was no clinical sign of toxicity on the eye, respiration system, and behavior up to 4000 mg/kgBW. At that dose, the extract did not cause mortality, lower the body weight, and lower the organ weight.