p-Sinefrin has lipolytic activity, however it has a low oral bioavailability, and also hydrophylic characteristic which is difficult to penetrate the epidermis if it is made into transdermal peparation as anti cellulite gel. The purposes of this research were to increase the penetration of p-sinefrin by preparing into transfersome gel. In this research three transfersome formulas were prepared, e.g. F1, F2 and F3 with the use of surfactants respectively: tween 80, span 80, and combination of tween 80 and span 80 with ratio 1:1. The result showed that F1 is the best formula with the highest entrapment efficiency 64.058 ± 0.754%, particle size average 103.3 nm , polydispersity index 0.269 ± 0.05 and zeta potential = -36.2 ± 0.64 mV, so the best formula was incorporated into gel formulation. There were two gel formulas prepared in this research, gel transfersome (GT) and non transfersome gel (GNT). Both of gels were evaluated for their physical stability, and also in vitro penetration test using Franz diffusion cells using rat male Sprague Dawley skin. The result showed the physical stabilty test of GT was more stable than  GNT. Cumulative penetration of p-sinefrin GT was higher than GNT, which value for GT was 1955.4± 9.36 µg.cm^-2 and GNT was 892.87 ± 31.79 µg.cm^-2. It can be concluded that GT can increase penetration of p-sinefrin compared to GNT.

Keyword : transfersome, gel, p-sinefrin, transdermal, penetration in vitro test