Snakehead fish (Channa striata) has been reported can be used for wound healing by people in South Borneo because contains albumin. Snakehead fish (Channa striata) extract has hydrophillic property and poor stability. Nanoparticle technology has been started to be developed as an alternative solution to improve drug delivery profile. The purpose of this study was to determine the formula that obtained best characterization for albumin nanoparticles. Albumin nanoparticles were prepared by ionic gelation method, that it was prepared by doing optimize ratio between snakehead fish extract : chitosan and pH of chitosan solvent.Albumin nanoparticles were characterized using Particle Size Analyzer for particle size and particle size distribution, measurement of entrapment efficiency, determined Zeta potential using Particle Size Analyzer, and observation of particle’s morphology using Transmission Electron Microscope. The result showed that the chosen formula was formula 6 which  ratio of extract : chitosan 1:2 with chitosan solvent pH 3, that formula 6( pH 3) had particle size 152.3 nm, polidispersity index 0.778, percentage of entrapment efficiency 51.3961 %, Zeta potential +35.9 mV, and round shape.

Keywords: ionic gelation, nanoparticle, snakehead fish(Channa striata)