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Acute Respiratory Infection (ARI) is an acute infection which attacks the respiratory tract which is caused by virus or bacteria. The high prevalence of ARI in Indonesia influences the use of antiinfection as therapy of ARI at healthcare facilities. Therefore, the aim of this study is to evaluate the use of antiinfection on ARI patients. This research was descriptive analytic which was done retrospectively at three primary healthcares in Depok during 2015. Samples consisted of 20441 ARI patients’ prescriptions containing antiinfection. Quantitative antiinfection use was expressed in Defined Daily Dose (DDD)/1000 patients/day unit, meanwhile the quality antiinfection use was assessed in type of antiinfection that accounted for 90% of the use (Drug Utilization 90%), and the adherence to national formulary. The result showed that the quantity of antiinfection used in Cipayung Primary Healthcare was 0.9496 DDD/1000 patients/day, Limo Primary Healthcare was 0.7590 DDD/1000 patients/day, and Bojongsari Primary Healthcare was 0.6483 DDD/1000  patients/day. The most commonly used antiinfection in Limo Primary Healthcare were amoxicillin, cotrimoxazol, and cefadroxil, while at Cipayung and Bojongsari Primary Healthcare was amoxicillin. The adherence percentage of the national formulary at Bojongsari Primary Healthcare was 71.43%, at Limo Primary Healthcare was 70.00% and at Cipayung Primary Healthcare was 63.64%.