LIPOXYGENASE INHIBITIORY ASSAY OF Averrhoa carambola L. LEAVES EXTRACT

Nabilah¹, Berna Elya¹, Djajadisastra Joshita¹

¹ Faculty of Pharmacy University of Indonesia

Keywords: Apigenin, Averrhoa carambola L., Lipoxygenase, LOX.

ABSTRACT:

Lipoxygenase (LOX) is one of the enzymes involved in the mechanism of inflammation. Flavonoid compounds have been studied to inhibit the inflammatory pathway. Apigenin is member of flavonoids that act as anti-inflammatory compounds. The leaves of plants Averrhoa carambola L. (sweet star fruit) has been known to contain apigenin. This study was conducted to test the ability of A. carambola L. leaves as a natural ingredient that plays a role in inhibiting inflammation. The research using in vitro LOX enzyme inhibition method. A.carambola L. leaves extracted using 70% ethanol, and fractionated with hexane, ethyl acetate and water. Each test sample testing LOX enzyme inhibition at pH 9, and linoleate acid as substrate. The product was messured using spectrophotometer at wavelength 234 nm. The control comparison using apigenin. The results showed that ethyl acetate fraction may inhibit LOX best with IC₅₀ value of 7.84 ± 0.03 ppm compared to other samples, 70% Ethanol Extract, water fraction and Hexane fraction with IC₅₀ values ​​in a row 37.00 ± 0.58, 64.09 ± 1.97 and 107.71 ± 2.02 ppm. IC₅₀ value of apigenin as a positive control showed IC₅₀ 2.03 ± 0.831 ppm.