Universitas Indonesia Conferences, 1st International Conference on Advance Pharmacy and Pharmaceutical Sciences

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Effect of Gamma Irradiation in Peperomia pellucida (L.) Kunth on Inhibition of Angiotensin Converting Enzyme (ACE) Activity
Fitria Ramadhani Ramadhani, Abdul Mun'im, Arif Arrahman

Last modified: 2016-07-27


Gamma irradiation is an effective technique can be used to reduce contaminants in herbal products. Peperomia pellucida (L.) Kunth belongs to piperaceae family has activity as ACE inhibitor. The aimed of this research were to determine the effects of gamma irradiation on microbial load, ACE inhibition activity and change of chemical compounds of  P. pellucida (L.) Kunth). Sample was irradiated at various dose of  0, 2.5, 5, 7.5 and 10 kGy and extracted by reflux method using 80% ethanol for 30 minutes. Microbial load was counted by using petrifilm aerobic count plate method. The ACE inhibition activity was tested by using ACE kit WST and change of chemical compounds, was examined by using thin layer chromatography profiling, used silica gel f254 plate as stationary phase and dichloromethane-methanol (90:10) mixture as eluent. Gamma irradiation up to 7,5 kGy reduced microbial load up to 2x102±0,00 cfu/g. ACE inhibition activity significantly increased (p<0,05) after irradiation treatment, but gamma  irradiation caused degradation of flavonoid content in Peperomia pellucida (L.) Kunth.