In vitro studies have proven that ginger (Zingiber officinale Rosc.) And sappan wood (Caesalpinia sappan L.) have antithrombotic activity. This study aimed to prove efficacy of the combination of both these plants by doing in vivo antithrombotic activity test with bleeding time and survival rate as the parameters. Sixty-six (66) mice were divided into two experimental groups (bleeding time and survival rate). The treatment groups consisted of normal control (CMC), negative control (CMC), positive control (Aspirin), and extract groups divided into dose 1, 2, and 3. All substances were administered orally for 7 days. For the bleeding time experimental groups, bleeding time was observed on mice tail that had been cut. For the experimental groups of survival rate, thrombosis induction was done by injecting collagen – epinephrine intravenously, then calculation of survival rate was performed. Results showed that bleeding time of mice in dose 2 group that was given ginger – sappan extract at dose 56 mg : 14 mg/20 g BW increased significantly (p ≤ 0.05) compared with the normal control. Dose 2 group also has higher survival rate than the negative control. Based on these results, it can be concluded that the combination of ginger – sappan extract at dose 56 mg : 14 mg/20 g has a potential as antithrombotic drugs because it can increase bleeding time and survival rate.

Keywords: Antithrombotic, ginger, sappan wood, in vivo, bleeding time, survival rate