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Excessive maternal fructose intake during pregnancy and in early postnatal life has metabolicconsequences for the offspring. We investigated the effects of melinjo (Gnetum gnemon) extract(MeE) intake during lactation on the expression and phosphorylation of adenosine monopho-sphate-activated protein kinase (AMPK) in the liver of offspring from excessive fructose-fedpregnant dams. Pregnant Wistar rats received a normal diet and 100 g/L fructose solutionduring gestation ad libitum. At delivery, dams were divided into two groups: a control diet (FC) ora 0.1% MeE-containing diet (FM) fed during lactation. The dams that were not treated withfructose were fed a control diet (CC). At postnatal week 3, some pups were sacrificed, while theremaining continued to receive a normal diet and were sacrificed at week 17. Blood chemistryand phosphorylation levels of AMPK and acetyl-coenzyme A carboxylase (ACC) were evaluat-ed. Plasma glucose levels in FC female offspring increased compared to that receiving CC atweeks 3 and 17; however, the levels in FM female offspring decreased at week 17. The insulinlevels in FM female offspring decreased significantly compared to that in FC female offspring atweek 3. Hepatic AMPK phosphorylation was upregulated in FM offspring at week 3 and infemale, but not male, offspring at week 17. ACC phosphorylation in FM female offspring wasupregulated at week 17. Our results suggest that maternal MeE intake during lactation maymodulate the hepatic AMPK pathways in female offspring.# 2016 Society for Biology of Reproduction & the Institute of Animal Reproduction andFood Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Sp. z o.o. All