SYNTHESIS AND CYTOTOXIC ACTIVITY EVALUATION OF GALLIC ACID DERIVATIVES IN HeLa CELL

ULFA, D.M¹, RADJI, M¹. ARSIANTI, A².

1Faculty of Pharmacy, University of Indonesia, Depok, Jawa Barat

2Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jalan Salemba Raya 6, Jakarta 10430, Indonesia

ABSTACT

Cervical cancer is the most common cancer among women in Indonesia. Gallic acid is a lead compound that has been proven have anti-cancer activity in vitro. The aims of this research are to synthesize, and evaluate the cytotoxic activity of gallic acid derivatives in HeLa cells. Gallic acid derivatives are designed  with subtitution of hidroxyl groups with O-propyl, O-alil, O-benzil and O-phenyl ethyl. Three gallic acid derivatives synthesized by condensation reaction with an alkyl halide, while the other two derivatives were synthesized by esterification reaction using Diisopropyl carbodiimide (DIC) and Dimethyl aminopiridine (DMAP) catalysts. The synthesized product identified by FT-IR, GC-MS-Spectrometer, ¹H-NMR and ¹³C-NMR. Cytotoxicity  evaluation are then done by MTT methode. It shows that three derivatives exhibited as a greater anticancer activity against HeLa Cell cells than the lead compound gallic acid with IC₅₀ 30,20 μM for benzyl gallate, 31,09 for propyl galtae and 34,58 μM phenyl ethyl gallate.

Key Words                            : gallic acid, esterification, cytotoxic evaluation