Wa Ode Yuliastri¹, Muhammad Isrul¹, Ahmad Saleh¹, Mus Ifaya¹

¹Department of Pharmacy STIKES Mandala Waluya Kendari, Southeast Of Sulawesi, Indonesia

Corresponding author: Waodeyuliastri@yahoo.com

ABSTRACT

Background: Sedative-Hypnotic are drugs that can depress the central nervous system. The use of Sedative-Hypnotic drugs can cause adverse side effects. Therefore, research using medicinal plant continues to be developed. Cananga odorata flowers are often used as traditional medicine. Objective: The purpose of this study was to determine the sedative-hypnotic activity of C.odorata extract. Materials and Methods : C.odorata flower was extracted by maceration method using 96% ethanol. Sedative-Hypnotics assay using mice (Mus musculus) is divided into 5 groups;  P1 was the negative control group (Na.CMC 1%), P2 was positive control (diazepam 2 mg), P3 was given C.odorata extract at 500 mg / 20 g BW mice, P4 was given C.odorata extract at 250 mg / 20 g BW mice, and P5 were given C.odorata extract  at 125 mg / 20 g BW mice. This research was conducted in two methods, The rotarod method to evaluate the sedative effect, the parameter observed is the length of time the mouse rotates at the rotarod and the righting reflex mice method, parameters observed were the time of sleep induction (onset) and the duration of sleep of mice. The data obtained were analyzed by One-way Analysis of Variance (ANOVA). Results:  all extract concentrations showed significant differences (p <0.05) when compared to negative controls (Na.CMC 1 %) which showed extracts had Sedative-Hypnotic activity. The extract at the concentration of 500 mg / 20 g BW mice did not show a difference with positive control (Diazepam 2 mg) Conclusion: Based on these results, C.odorata extract has a potential effect as an Sedative-Hypnotic agent.

Key words: Sedative-Hypnotic, Cananga odorata, Rotarod, Righting reflex