ABSTRACT

Background: Mangosteen peel (Garcinia mangostana)L., binahong leaf (Anredera cordifolia (Ten.)), and centella herb (Centella asiatica) have been shown to have activity in each phase of burn healing process.  Formulating three plant extracts into cold cream preparations is expected to increase the healing activity of burns. Objective: This study aimed to prove the efficacy of the cold cream combination of these plants by examining in vivo burn healing activity by employing a deep second-degree burn rat model. Materials and Methods: Rat were divided into nine experimental groups (normal, saline solution, silver sulfadiazine, cold cream base, each three extracts, combination of extract, and coldcream combination of three extract). The burn activity was observed by measuring the percentage of wound healing by visual and assessed inflammatory cell, agiogenesis and collagen formation through histopathological examination. Results: Cold cream combination of mangosteen peel., binahong leaf, and centella herb enhanced the burn wound healing as indicated by significant increased percentage of wound healing compared with all grup (p ≤ 0.05). Histological result showed that coldcream combination group can reduced inflammatory cell infilration and increased collagen significantly (p ≤ 0.05) , the other hand same as normal control, but has no effect on agiogenesis. Conclusion: Based on these results, cold cream combination of mangosteen peel., binahong leaf, and centella herb enhanced has a potential effect as an burn healing agent.

Key words: Burn healing, cold cream, mangosteen peel, binahong leaf, centella herb