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Background: Bengle (Zingiber cassumunar)  is one of the plants that has the potential as an immunomodulator. Bengle rhizome activity as an immunomodulator can be observed through curcumin compounds contained in Bengle which can increase the phagocytic activity of macrophages and lymphocyte proliferation. Objective: This study aims to examine and determine the effect of ethyl acetate fraction extract of bengle on phagocytic activity of macrophages and lymphocyte proliferation in vitro. Materials and Methods: The Bengle rhizome is macerated with 96% Ethanol solvent and then followed by ethyl acetate fractionation. This study used male Balb / C strain mice macrophages divided into 4 groups: control group, 25 concentration group, 50 concentration group, and 100 μg / mL concentration group. Results: The results showed that ethyl acetate fraction of bengle extract can increase the phagocytic activity of macrophages seen from the parameters SFA (active phagocytes cells) and phagocytic index. The highest percentage of SFA and phagocytic index results were at a concentration of 100 μg / mL. The results showed that ethyl acetate fraction of bengle extract could increase lymphocyte proliferation with a significance value of p <0.05. This is seen from the results of the highest SI Proliferation located at a concentration of 25 μg / mL.  Conclusion: it can be concluded that the administration of ethyl acetate bengle extract (Zingiber cassumunar Roxb.) has the potential as an immunomodulator