Comparison of Urinary Hydrogen Peroxide (H₂O₂) Level in Type 2 Diabetes Mellitus Patients with estimated GFR ≥ 90 and 60-89 mL/min/1,73 m²

Nadya Religiane Aretha, Azizahwati, Rani Sauriasari^*

Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.

*Corresponding author: rani@farmasi.ui.ac.id

ABSTRACT

Background: One of the chronic complications in patients with type 2 diabetes mellitus is kidney dysfunction which is known as diabetic nephropathy. One marker that has been standardized as a detector of kidney damage is the estimation of the glomerular filtration rate (eGFR). On the other hand, hydrogen peroxide is also being investigated for its role as a marker of kidney damage. Objective: This study aims to compare the urinary hydrogen peroxide levels in the group of patients with diabetes mellitus with eGFR ≥90 and 60-89 ml/min/1,73 m². Materials and Methods: The study was conducted using a cross sectional study and purposive sampling technique with a sample of 196 subjects taken from Dr. Sitanala Tangerang and Pasar Minggu District Health Center. The eGFR value is obtained from the CKD-EPI equation and the urinary hydrogen peroxide value is obtained from a measurement using the Ferrous ion Oxidation Xylenol Orange method (FOX-1) normalized by urine creatinine. Results: The mean difference of urinary hydrogen peroxide levels in the eGFR ≥90 mL/min/1,73 m² and in the eGFR 60-89 mL/min/1,73 m² was showed no significant difference (p=0,946). In addition, the mean comparation of urinary hidrogen peroxide level in eGFR group ≥90 mL/min/1,73 m² and eGFR 60-89 mL/min/1,73 m² with normoalbuminuria and albuminuria showed significant difference (p=0,002) Conclusion: Based on these results, there was no significant difference in urinary hydrogen peroxide level with estimated glomerular filtration rate.

Key words: Diabetes mellitus, nephropathy diabetic, oxydative stress, hydrogen peroxide, eGFR