Comparison of Serum OxLDL-β2glycoprotein I Complex in Type 2 Diabetes Mellitus Patients with Estimated Glomerular Filtration Rate ≥ 90 and 60-89 mL/min/1,72 m²

Afina Irsyania Zulfa, Azizahwati, Rani Sauriasari*

Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.

*Corresponding author: rani@farmasi.ui.ac.id

ABSTRACT

Background: Diabetic nephropathy is a condition that refers to structural and functional damages to the renal and is one of the main causes of end-stage renal failure. The key mechanism that is thought to be involved in the pathogenesis of diabetic nephropathy is oxidative stress. Reduction of the glomerular filtration rate can precede the development of microalbuminuria that now the diagnosis of diabetic nephropathy is also based on the value of eGFR. One marker of oxidative stress is the oxLDL-β2 glycoprotein I complex that participates in glomerulosclerosis and interstitial fibrosis. Objective: This study aimed to compare the serum levels of oxLDL-β2glycoprotein I patients type 2 diabetes mellitus with eLFG values calculated using the CKD-EPI equation to assess the association with the pathogenesis of diabetic nephropathy. Materials and Methods: Samples were divided into two groups, which was patients with eGFR ≥ 90 ml/minute/1.73 m² (n = 118) and patients with eGFR 60-89 ml/minute/1.73 m² (n = 62). Serum oxLDL-β2 glycoprotein I complex level was measured by ELISA. All measurements were done in 2015 at RSK. Dr. Sitanala, in 2016, and 2019 at Pasar Minggu Community Health Center. Results: The mean difference of serum oxLDL-β2 glycoprotein I complex with eGFR ≥ 90 ml/minute/1.73 m² and patients with eGFR 60-89 ml/minute/1.73 m² was showed no significant difference (p=0.262). In addition, the mean comparison of serum oxLDL-β2 glycoprotein I complex levels in the eGFR group ≥ 90 ml/minute/1.73 m² and 60-89 mL/minute/1.73m² with normoalbuminuria and albuminuria also showed no significant difference (p = 0.071). Conclusion: There was no significant difference in serum oxLDL-β2 glycoprotein I complex level with estimated glomerular filtration rate and the results of the analysis indicate that the serum oxLDL-β2 glycoprotein I complex is not specific enough as a marker of early diabetic nephropathy.

Key words: diabetic nephropathy, eGFR, oxidative stress, type 2 diabetes mellitus, oxLDL-β2 glycoprotein I complex