Intranasal drug delivery is an effective drug route for absorption into the systemic circulation or transported directly to the brain. The surface area of the nasal mucosa can provide fast onset of therapeutic effect, direct delivery to the central nervous system, and is not invasive so that it can improve patient comfort in carrying out therapy. To increase the penetration of valproic acid can be done by formulating the medicinal ingredients into a nanocarrier system such as liposomes. Valproic acid encapsulated with liposomes was tested for diffusion properties with and without encapsulation of liposomes through sheep's nasal mucous membranes. Valproic acid liposomes are made by the thin layer hydration technique with phosphatidylcholine acid and cholesterol. Optimization of liposome encapsulation is best at the ratio of the molar ratio of phosphatidylcholine: cholesterol: valproic acid is 2: 1: 0.1. In measuring the temperature conditions of 25 ° C, the average particle diameter size of valproic acid liposomes is 271nm, PDI (Polydispersity index) value is 0.583, and potential zeta value is -50.3. Furthermore, valproic acid liposomes were tested penetrating with sheep mucous membranes. The results of this study were to obtain valproic acid liposome preparations that met the requirements for intranasal administration.