Universitas Indonesia Conferences, Asian Federation for Pharmaceutical Sciences (AFPS) 2019

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Antimalarial Activity of Ethyl Acetate Fraction and Tablet (AS201-01) of Andrographis paniculata in Plasmodium berghei infected mice
Lidya Tumewu, Dery Astrianto, Dwi Ayu Fitrianingtyas, Hilkatul Ilmi, Indah Setyawati Tantular, Endang Widiastuti, Achmad Fuad Hafid, Aty Widyawaruyanti

Last modified: 2019-07-13


Background: Andrographis paniculata was reported to have various pharmacological activities. It is well known as antimalarial agent wherein diterpene lactone compounds take a role on the activity. Based on that fact, A. paniculata was fractionated to obtain ethyl acetate fraction which rich of diterpene lactone content. Regarding to its potential activity, ethyl acetate fraction was developed as tablet dosage form namely AS201-01. Formulation process influenced the activity of tablet. Therefore, antimalarial activity of ethyl acetate fraction and AS201-01 tablet need to be determined. Objective: This study aims to determine antimalarial activity of ethyl acetate fraction and AS201-01 tablet of Andrographis paniculata. Materials and Methods: In vivo antimalarial assay was done based on Peter’s four days suppressive test. Plasmodium berghei infected mice were divided into nine groups. Group one as control was given Carboxylmethyl cellulose-Natrium (CMC-Na) 0.5% twice a day for four days. Group two-five were treated with ethyl acetate fraction. Group six-nine were treated with AS201-01 tablet. Ethyl acetate fraction and AS201-01 tablet was administered twice a day for four days at a dose equal to andrographolide of 6.25, 12.5, 25, and 50 mg/kg BW. P.berghei growth was observed for seven days and compared to control group. Meanwhile, survival time was observed for 14 days. Results: Ethyl acetate fraction and AS201-01 tablet were exhibited antimalarial activity with Effective Dose 50% (ED50) value of 4.21 mg/kg body weight and 5.95 mg/kg body weight, respectively. Survival time of treatment group was 12-13 days. Meanwhile, survival time of control group was 10 days.  Conclusion: The results revealed that ethyl acetate fraction had higher antimalarial activity compared to AS201-01 tablet. Therefore, further formulation study need to be continued. Both of ethyl acetate fraction and AS201-01 tablet were increased survival time compared to control group.