Universitas Indonesia Conferences, Asian Federation for Pharmaceutical Sciences (AFPS) 2019

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Functional Group Analysis of Solvent-Partitioned Extract of Eleusine indica and its ACE Inhibitory Activity
Joanne Katherine Talens Manlusoc, Jade Celon Cometa, Liahona Faith Roldan Mancol, Anthony Nalzaro Yen

Last modified: 2019-07-12


Background: Eleusine indica Linn. Gaerth or Indian goose grass has been widely used as an alternative natural anti-hypertensive agent. However, the rationale behind this mechanism is still unknown. Objective: The purpose of this study is to confirm presence of functional groups that may be responsible to its ACE inhibitory activity and its possible synergism. Materials and Methods: Each solvent-portioned fraction was analyzed by FTIR, individual ACE inhibitory activity, and combination of fractions. Results: FTIR results of solvent-partitioned fractions revealed that O-H and C-H stretches were present in all fractions. In addition to this, C-O and C=O groups were also present in all fractions except in ethyl acetate. S=O groups were also present in chloroform, ethyl acetate, and n-butanol fractions. Other function groups such as N-H and C=C groups were present in ethyl acetate and n-butanol fractions respectively. The highest % inhibition obtained among the individual fraction is n-butanol at 96.031 % ± 0.004 at 500 ug/ml. The lowest % inhibition obtained among the individual fraction is chloroform at 32.544 % ± 0.011 at 500 ug/ml. The highest % inhibition among the combined fractions is ethyl acetate:butanol at 95.727% ± 20.847, while the lowest % inhibition in combination is hexane:chloroform at 89.626 % ± 19.577. The fraction with the lowest IC50 is hexane at 15.357, while the highest IC50 is chloroform at 168.207 ug/ml. Conclusion: Functional group present may be associated with the antihypertensive activity of the E. indica extracts. The more polar solvent-partitioned fractions of E. indica has a higher ACE inhibitory activity compared to its lesser polar solvent-partitioned fractions. The higher ACE inhibitory activity may be associated with the secondary metabolites present in the more polar fractions. There is also possible synergism present in the combined solvent-partitioned fractions as their % inhibition is higher than their individual effects.

Key words: Eleusine indica, paragis, ACE inhibition, FTIR, ACE kit-WST