Background: Blueberry is a native fruit from North America that attracts a lot of attention for their antioxidant effect. This fruit is widely consumed in Indonesia and known for their benefit in health.  Several studies have proven that blueberry has protective effect for degenerative disease, vascular diseases, and cancer. The pathophysiology of these diseases is stress oxidative that can be reduced by supplementation of antioxidant. Objective: This study aims to determine the antioxidant and anti-cancer potential of blueberry. Materials and Methods: Frozen blueberry (Vaccinium corymbosum L.) was extracted with ethanol using maceration technique. Extraction result were tested for phytochemical screening, total antioxidant capacity (Blois), total phenolic contents (Singleton & Rossi), total flavonoid contents (Woisky and Salatino), and toxicity (Meyer). Results: Phytochemical screening showed positive results on alkaloids, phenols, anthocyanin, betacyanin, glycosides, cardio glycosides, coumarins, flavonoids, quinones, steroids, terpenoids, and tannins, total antioxidant capacity IC₅₀ 42.90 μg/mL (very strong), total phenolic contents 967.69 µg / mL, total flavonoid contents 8.012 µg/mL, and toxicity value LC₅₀ 126.765 µg/mL (toxic). Conclusion: Based on these results, blueberry has potency to be used as antioxidant and anti-cancer.