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Background: Binahong is a plant that has a potential to be used as a traditional herbal medicine in Indonesia, and has several kinds classes of compounds, one of them is flavonoids glycosides (vitexin). Previous researchers reported that binahong leaves have pharmacological activities as antihyperglycemic, antihyperlipidemic, antibacterial and others. A traditional plant that has proven efficacious needs to be standardized to ensure the quality and its safety. Objective: The aim of this study was to determine the characteristics of binahong leaves simplicia obtained from Bogor, West Java. Materials and Methods: The crude extract was obtained by maceration method using 40%, 70% and 96% ethanol solvent. The selected extract is then standardized which includes macroscopic and microscopic observations and sets the standard parameter values binahong leaf extract.Parameters LCMS to identify active compounds semi quantitatively. Results: The yield of binahong ethanol extract from 40%, 70%, 96% showed a value of 10.9%, 11.4% and 12.32% respectedly, from this results 96% ethanol extract was proceeded for standardization. Macroscopic observation results showed that binahong leaves simplicia has a pointed and notched  form with 5-10 cm long and 3-7 cm diameter.The microscopic binahong leaves contains palisade tissue, parenchymal tissue, chlorophyll grains, rosette Ca-oxalate crystals and spiral type. Phytochemical screening of binahong leaves showed the presence of alkaloids, flavonoids, saponins, tannins, steroids and phenolic compounds.The standardization of binahong leaves ethanol extract down showed a levels of ethanol soluble extract> 14.8%, water soluble extract content > 13.5%, drying < 10%, water content < 8.9%, total ash content < 7.2%. LCMS profiles showed that ethanolic extract 40%, 70% and 96% all contained vitexin at retention time 5.02 minutes and m/z values ​​433.1111. Conclusion: 96% ethanolic extract of binahong leaves contains vitexin with pharmacognosic parameters carried out in accordance with the standards listed in the Indonesian herb pharmacopoeia.

 

Keywords: Anredera cardifolia, Standardization, Simplicia, Extract, LCMS