Ratih Dyah Pertiwi¹*, Joshita Djajadisastra², Abdul Mutalib³, Anung Pujiyanto⁴

¹Department of Pharmacy-Universitas  Esa Unggul, Jakarta, Indonesia

²Department of Pharmaceutical Science Faculty of Pharmacy-Universitas Indonesia, Depok, Indonesia

³Department of Chemistry Faculty of Mathematics and Natural Sciences-Universitas Padjajaran, Bandung,  Indonesia

⁴Center for Radioisotope and Radiopharmaceutical  Technology-National Nuclear Energy, Tangerang Selatan,  Indonesia

*ratih.dyah@esaunggul.ac.id

Abstract

Gold nanoparticles (GNPs) have recently developed as an attractive candidate for delivery of various payloads into their targets  They are attractive for cancer applications as they can be engineered to have multifunctionality serving simultaneously as imaging contrast agents, therapeutic agents, and/or drug delivery vehicles. GNPs are prepared by the reduction of gold in presence of a stabilizing agent which prevents the particle agglomeration. Coated GNPs  with conjugated gum arabic (GA) as a stabilizing agent and vincristine (VCR) as therapeutic agents were successfully synthesized and characterized. The conjugation of GA-VCR and GNPsdisplayed a narrow hydrodynamic particle size distribution with average size <100 nm by Transmission Electron Microscopy (TEM) and Particle Size Analyser (PSA).We investigated the cytotoxic activity of conjugated vincristine-gum Arabic-gold nanoparticle (VCR-GA-GNPs)  by tetrazolium salt assay (MTT) using cancer cell line MCF-7. Cytotoxic activity of conjugated VCR-GA-GNPsbefore and after purification by Size Exclusion Chromatography (SEC), against cell line MCF-7 was described by IC50 value. All formulation had a cytotoxic activity with IC50 <20μg/ml. The IC50 of samples against MCF-7 cell line were 3,59 μg/ml and 3,10 ug/ml, respectively.

Keywords: Gold Nanoparticles, MCF-7, Vincristine, Gum Arabic, cytotoxic activity