Background: Utilization of Aloe vera in the cosmetic industries has been receiving increased interest and attention as of lately. Despite its popularity, most Aloe vera products available in the market are monophasic gel system with limited capability of including lipid-soluble components, thus providing the potential of developing various novel system with enhanced characteristics. Objective: This study aimed to formulate novel microemulsion system containing freeze-dried Aloe vera extract, tocopherol acetate and various plant oils (Sunflower, Olive, Grapeseed and Coconut oil) with varying unsaturated fatty acid composition and evaluate its effect on the penetration-enhancing capacity and free-radical scavenging activity. Materials and Methods: Microemulsion containing different plant oils, tocopherol acetate and freeze-dried extract were prepared using high-speed homogenizer and their pH, viscosity and distribution of globule size were measured. Additionally, the penetration enhancing effect of the plant oils were evaluated by in vitro diffusion method using Franz diffusion cell and their free-radical scavenging activity were determined using DPPH (1,1,-diphenyl-2-picryl-hydrazil). Results: Microemulsion were obtained and significant differences of penetration-enhancing capacity as well as free-radical scavenging activity were observed for different microemulsion formulation among various plant oils with varying fatty acid composition. Conclusion: This study revealed that plant oils could be used as promising alternative to be utilized as natural penetration enhancer of tocopherol acetate in a microemulsion system containing aloe vera extract.