The ethanolic extract of Javanese turmeric rhizome  possesses curcuminoid and xanthorrhizol. The extract have been believed to show  anti-diabetic activity which can further be improved by the The designs of nanoparticles preparation. The aim of this study was to compare the anti-diabetic activity of the nanoparticles containing the ethonolic extract of Javanese turmeric rhizome and its tablets. The rhizome was extracted using maceration with ethanol 96% (v/v) and was then formulated into nanoparticles with chitosan as a matrix. The tablets were prepared by direct compression method. In the study of anti-diabetic activity, the mice were divided into 4 treatment groups, namely group I as a negative control, group II as a positive control, group III treated with the nanoparticles (400 mg/Kg BW), group IV treated with the tablets (400 mg/Kg BW). All test substances were  orally administered for 14 days. The datas of body weight and blood glucose level were recorded on day 0 after acclimatization, day 5 after alloxan induction for 4 days, day 12  and day 19 (7 days and 14 days after treatments with each test substance). The results of the analysis of glucose levels demonstrated the decrease in blood glucose for both nanoparticles and the tablets. The results of The percentage of the decrease were 26.33% for positive control, 24.59% for the nanoparticles, and 23.13% for the tablets. Accordingly, the tablet formulation did not influence the anti-diabetic activity of the nanoparticles.