In recent years, studies have shown that there is a correlation between sleep disorders and cancer, but at the present stage, the research on sleep disorders and tumor related animal models is relatively insufficient. Our research will focus on mice bearing B16F10-luc-G5 melanoma tumor with sleep fragmentation, detecting promoting effect of sleep fragmentation (SF) on the metastasis of melanoma. At the same time, we used Ganoderma lucidum polysaccharides peptide (Gl-pp, 80mg/kg), a component of traditional Chinese medicine Ganoderma lucidum, which has long enjoyed a good reputation at home and abroad, to observe its anti-tumor metastasis effects on B16F10-luc-G5 mice with SF. Then we used whole proteomics to analyze the difference proteins expressed in lung tissue and compared between groups, includes mice bearing B16F10-luc-G5, mice bearing B16F10-luc-G5 with SF and GL-pp administered mice bearing B16F10-luc-G5 with SF. With the analysis using bioinformatics, we found several key proteins, their genes name are Adcy9, ptk2, Yap1 and Lpin2, Per1 and Tim. And several important clusters, they are, immune system, platelet aggression, energy metabolism, cell cytoskeleton, cell adhesion and circadian rhythms. Moreover, we detected the TLR4 signal pathway and macrophage differentiation to reconfirm the results of proteomics and trying to elucidate the mechanism of SF on tumor growth and metastasis and the effects of GL-pp.

Key words: Ganoderma lucidum polysaccharide peptide, Fragmented sleep, Melanoma, Proteomics, Tumor immunity