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Objective. This study aimed to get complete information about the antioxidant and antibacterial against B. subtilis and Staphylococcus aureus activities of the fractions from G. latissima Miq. stem bark methanol extract. Methods. Fractionation was performed by column chromatography. The antibacterial activities of the fractions from Garcinia latissima Miq. stem bark were assayed by inhibition zone technique, bioautography, and minimum inhibition concentration. The antioxidant activity were evaluated using DPPH (2,2-diphenyl-1picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) methods. Results. Stem bark methanol extract had higher antioxidant activity (% inhibition = 95.68%) than ethyl acetate extract and n-hexane stem bark extract of G. latissima Miq. The stem bark methanol extract obtained ten fractions. The greatest of inhibition zone diameter against B. subtilis (ATCC (the American Type Culture Collection) 6633) and S. aureus (ATCC 25923) was fraction G (7.83 ± 0.46 mm and 7.43 ± 0.15 mm). The highest antioxidant activity fraction by DPPH method and FRAP method was fraction G. Phytochemical screening showed that fraction G contained flavonoid and tannin. Conclusion. The results obtained reveal that the fraction G of stem bark of G. latissima Miq. methanol extract possessed antibacterial activity against B. subtilis and S. aureus and antioxidant activity.